first_img 00:00 00:00 spaceplay / pause qunload | stop ffullscreenshift + ←→slower / faster ↑↓volume mmute ←→seek  . seek to previous 12… 6 seek to 10%, 20% … 60% XColor SettingsAaAaAaAaTextBackgroundOpacity SettingsTextOpaqueSemi-TransparentBackgroundSemi-TransparentOpaqueTransparentFont SettingsSize||TypeSerif MonospaceSerifSans Serif MonospaceSans SerifCasualCursiveSmallCapsResetSave SettingsESCONDIDO (KUSI) — Everyone knows we love our clean cars in Southern California. But Escondido could have the cleanest cars in San Diego County: 28 car washes and counting.“We just need to look at this closer. Do we really need any more car washes in town,” said Escondido City Manager Jeffery Epp.This came as a big shock to the local businessman who bought a piece of land, got most of it approved for a car wash, then the city pulled the plug.“We did the environmental studies, we did traffic studies, who did everything needed and I was waiting for final approval. Then they decided to impose a moratorium on me,” said Wash n Go owner Neil Capin.Capin invested a lot of money in the old skating rink, a place that has a lot of nostalgia, but it’s been shut down for 10 years.“If someone wanted to build a skating rink, they should have done it,” Capin said.After the city council voted in favor of the 45-days moratorium, Capin still has hope.“What I hope is that the projects that are already in the works keep moving forward. I was almost finished with the whole process, we should be able to finish the project,” Capin said.“I don’t think anyone is telling him he can’t build it, We just need a time out, take a look. Do we really need another car wash in Escondido,” Epp said.After investing hundreds of thousands of dollars into the skating rink, Capin is not pleased.“Why would anyone invest in Escondido, when something like this can happen. We should be allowed to “finish” the project. It’s just not right to start a project and have them change in midstream,” Capin said.A final decision is expected within the 45-day moratorium. Updated: 10:28 PM Too much of a good thing: Car wash surplus leads to drama Dan Plante, Categories: Local San Diego News FacebookTwittercenter_img Dan Plante May 2, 2018 Posted: May 2, 2018last_img read more

first_img Share your voice Random Post a comment 13 Photos Bill Gates Bill Gates has some suggestions for your 2019 summer reading list. Bill Gates/Gates Notes Summer vacation is almost here in the northern hemisphere. Woo-hoo! You’re heading to the beach. You could pack a romance novel or a lightweight spy caper. Or you could pack the five books Microsoft founder Bill Gates recommends you read. Get ready to get heavy.Gates’ summer reading list is an annual tradition. He released the latest version on his Gates Notes blog on Monday, giving you plenty of time to hit your local bookstore for some thought-provoking reading to pack with your sunscreen and bathing suit.”I’ve recently found myself drawn to books about upheaval (that’s even the title of the one of them) — whether it’s the Soviet Union right after the Bolshevik revolution, the United States during times of war, or a global reevaluation of our economic system,” Gates wrote, saying most of these books deal with the idea of disruption. 0 UpheavalJared Diamond’s Upheaval delves into “how nations managed existential challenges like civil war, foreign threats and general malaise” through a group of case studies. Gates acknowledged this might sound like a bummer, but said he left the book feeling optimistic about our ability to solve problems.Nine PintsIf you made it through all the bloodshed of Game of Thrones, then chances are good you can handle Nine Pints by Rose George. The subtitle clues you into the subject: “A Journey Through the Money, Medicine, and Mysteries of Blood.” “It’s filled with super-interesting facts that will leave you with a new appreciation for blood,” Gates said.A Gentleman in MoscowNow we detour into the realm of fiction with Amor Towles’ A Gentleman in Moscow. This bestselling novel follows the rich Count Alexander Rostov who is living under house arrest in a hotel in Moscow in the 1920s. The book spans several decades. Gates called it “fun, clever, and surprisingly upbeat.” Presidents of WarMichael Beschloss’ Presidents of War tackles the complicated history of US presidents and the conflicts the country became involved in, including the War of 1812 and the Vietnam War. “Beschloss’s broad scope lets you draw important cross-cutting lessons about presidential leadership,” said Gates.The Future of CapitalismGates said he doesn’t agree with everything in Paul Collier’s The Future of Capitalism, but that the author’s “background as a development economist gives him a smart perspective on where capitalism is headed.” Gates recommended this as a good analysis of the problems around capitalism today.While these five titles make up the official summer reading list, Gates also threw in a plug for his wife Melinda Gates’ The Moment of Lift, a book about the women who have inspired her. “I know I’m biased, but it’s one of the best books I’ve read so far this year,” he said. These books should keep you busy until the end of 2019 when we can expect Gates’ annual list of his favorite books of the year. 1:08 Tags How to check out digital library books Inside Microsoft’s HoloLens development lab Now playing: Watch this:last_img read more

first_imgSonakshi Sinha in Khandaani ShafakhanaPR HandoutBollywood actress Sonakshi Sinha bid an emotional farewell to the team of Khandaani Shafakhana after wrapping up its shoot and the latest video is now creating a lot of buzz on social media.Sonakshi Sinha has never shied away from playing diverse roles. From adorable Rajjo in Dabangg to kicking ass in Akira, she has done it all. In her next film Khandaani Shafakhana, she will be playing the role of a young girl from Amritsar. It is about how she inherits her uncle’s sex clinic and tries to break the taboos around the topic. The film, which is directed by Shilpi Dasgupta also stars Varun Sharma and Badshah.Sonakshi Sinha shared a video on her Instagram page and captioned it with, “And in बेबी Bedi’s style, bada changa experience thaa ji! Thank you @shilpidasgupta @mriglamba @fukravarun @badboyshah @priyanshjora @dprishi @gautam.m1 and the entire team for making @khandaanishafakhana one of my most cherished films!!! ❤See you at #KhandaaniShafakhana on 2nd August at your nearest cinema. #BaatTohKaro.”The video shows the making of some scenes. Sonakshi Sinha is also seen speaking about how Khandaani Shafakhana and the team are extremely special for her. Despite being her 23rd film, she knew everyone from the cast and crew, how the vibe on the sets was extremely warm, and this will be a special film for her.Sonakshi Sinha is the first female actor to headline a film which deals with the subject of sex, and she has been getting a lot of praise for how she is trying to encourage people to approach the subject openly. Apart from that, she has also received a positive response on how she has gotten into the skin of the character of Baby Bedi with such ease.Khandaani Shafakhana is scheduled to release on August 2. Two weeks later, Sonakshi Sinha will be seen playing the role of a scientist in Mission Mangal, which hits the screens on August 15.last_img read more

first_imgStatue of Supreme Court Chief Justice Roger Brooke Taney at the Maryland State House. Feb. 23, 2016. (Capital News Service photo by Connor Glowacki.)ANNAPOLIS — Legislation moving through Annapolis could put a statue of Supreme Court Chief Justice Roger Brooke Taney, a Maryland native known for writing the pro-slavery Dred Scott opinion, in jeopardy.Monuments from the Civil War with ties to slavery and the Confederacy have been an increasingly controversial matter throughout Maryland in the past year. Over 20 Democratic delegates are co-sponsoring a House bill that, if passed, would remove and destroy the statue. The bill is set to be introduced to the Maryland House Health and Government Operations committee on Wednesday and has a companion bill in the state Senate, set for a hearing March 4.One of the House bill’s sponsors, Delegate Benjamin Brooks, D-Baltimore County, said that growing up in South Carolina and being subjected to racial prejudices gave him a unique perspective on this issue. “I think any symbol of racial hatred and oppression should not be something the state is endorsing,” Brooks said. “Anything and everything I can do to remove them from society, I’ll do it.” Taney was the fifth chief justice in the history of the U.S. Supreme Court and is remembered most for his decision regarding the Dred Scott case in 1857. Scott was an enslaved African American who said that he and his wife should be granted freedom because they were taken as slaves, by a white owner, to live in Illinois and the Wisconsin Territory for four years. But both those states considered slavery illegal. According to the case’s decision on the Cornell University Law School website, Taney said in the opinion, “Neither Dred Scott himself nor any of his family were made free by being carried into this territory, even if they had been carried there by the owner with the intention of becoming a permanent resident.”Taney grew up in Calvert County and practiced law for over 20 years in Frederick County before becoming a part of the Supreme Court in 1836, according to the Historical Society of Frederick County’s website. Delegate Kathy Afzali, D-Frederick, disagreed with the bill and said there were more important things that the legislature could be doing this session. She also said that there were a lot of historical figures who had prejudices, but who were also in tune with their time.“I honestly think we have parts of our history that are good and then parts that are embarrassing, but he was still a part of our history,” Afzali said.Afzali said that instead of removing the statue, there should be a plaque added to the monument detailing Taney’s decision in the Dred Scott case. However, Brooks said he would consider amending the bill to move rather than destroy the statue.“Let’s put it in a library or something. Memorialize it that way,” Brooks said.last_img read more

first_img ×Actors Reveal Their Favorite Disney PrincessesSeveral actors, like Daisy Ridley, Awkwafina, Jeff Goldblum and Gina Rodriguez, reveal their favorite Disney princesses. Rapunzel, Mulan, Ariel,Tiana, Sleeping Beauty and Jasmine all got some love from the Disney stars.More VideosVolume 0%Press shift question mark to access a list of keyboard shortcutsKeyboard Shortcutsplay/pauseincrease volumedecrease volumeseek forwardsseek backwardstoggle captionstoggle fullscreenmute/unmuteseek to %SPACE↑↓→←cfm0-9Next UpJennifer Lopez Shares How She Became a Mogul04:350.5x1x1.25×1.5x2xLive00:0002:1502:15 “Xuanzang,” a co-production between Eros and China Film Group, was China’s unsuccessful submission to the foreign-language Oscar category in 2016. The two companies have a slate of other co-ventures.Operating both ad-supported and subscription models, iQIYI claims to have 500 million monthly active users and 60 million regular subscribers. It floated on NASDAQ earlier this year and currently enjoys a $19 billion market capitalization. Popular on Variety center_img Leading Bollywood distributor Eros International has struck a content-licensing deal in China with streaming giant iQIYI.Eros described the deal as a content-licensing pact for Eros Now, its own streaming video platform. In a statement, it said that “Eros Now becomes the first South Asian OTT player to make inroads into the Chinese digital space.” Details were not available.In recent weeks, Eros has announced more advanced partnerships that expand the operation of Eros Now in other territories. In both India and Indonesia, Eros Now will be carried as part of the Mi TV platform operated by Chinese phones and hardware group Xiaomi.Indian films have enjoyed a surge of popularity in theaters in China, with “Dangal,” “Secret Superstar,” and “Hindi Medium” all becoming hits. Other efforts are being made to establish co-production films between the two neighboring but often rival countries.last_img read more

first_imgIn the prokaryotic respiratory chain, the situation is more complicated. A complete pathway of electron flow has not yet been determined in the cell type due to its complexity. It is therefore necessary to understand the complete structure of a “supercomplex” involved during bacterial electron transfer to assist the goal. In the study, the researchers extracted and purified the complex from M. smegmatis to visualize the architecture using cryo-electron microscopy (cryo-EM) at a resolution of 3.5 Å. The structure provided crucial insights into the mechanism of direct electron transfer within a respiratory supercomplex. The dimensions of the supercomplex were in the range of 200 x 70 x 120 Å, in a symmetrized linear architecture completely different from previously reported respiratory supercomplexes. By composition, the linear dimeric CIV1-CIII2-CIV1 was arranged such that individual CIVs flanked the central CIII dimer on either side. The information revealed a direct link between enzymes during electron transfer, representing a new mode of respiratory chain catalysis. The detailed structural findings have potential to assist with antimycobacterial drug discovery efforts. The architecture of the supercomplex in its entirety: The cartoon representation shows the menaquinone/menaquinol (MK) in bright green colored solid spheres and phospholipids as yellow sticks. Credit: Science, doi: 10.1126/science.aat8923 The authors revealed the cryo-EM structure of a CIII-CIV respiratory supercomplex of the M. smegmatis bacteria. The intra-complex electron transfer pathway ranged from quinol oxidation in CIII to oxygen reduction in CIV. The results showed a new mechanism for bifurcating electron transfer to ensure completion of the Q cycle (the net movement of protons across a lipid bilayer) for energy transduction. The association of a superoxide dismutase in the architecture of the system can protect against oxidative damage by reactive oxygen species (ROS). The architecture of quinone binding sites also provided a framework for future studies in structure-based antimicrobial drug discovery. Study shows how bacteria guide electron flow for efficient energy generation In a recent study conducted by Hongri Gong and colleagues, a respiratory supercomplex was isolated from the bacterium Mycobacterium smegmatis, and its structure was visualized at a resolution of 3.5 Å using cryo-electron microscopy (cryo-EM). The bacterium is a close relative to M. tuberculosis and a popular model used to study many other bacterial species. The detailed structure revealed how electrons were transferred in the cell in a process hitherto unseen. Respiration in Actinomycetes and overall architecture of the Mycobacterial respiratory machine CIII2CIV2SOD2. A) The respiratory electron transfer chain in Actinomycetes (left) and the 5 major prokaryotic cytochrome c pathway variants with the organization schemes in representative organisms (right). The M. smegmas cytochrome c pathway has its cytochrome c fused with complex III to form a bcc-type complex III that interacts with the aa3-type complex IV to assemble into a CIII-CIV supercomplex. MK = menaquinone/menaquinol. B) The entire architecture of the bcc-aa3 type respiratory CIII-CIV Supercomplex from M. smegmatis. The cryo-EM map of the supercomplex shows a linear 2-fold dimerized form of CIV1-CIII2-CIV1 with dimensions 200 x 70 x 120 Å. CIII is seen in orange, CIV is magenta and the association factors PRSAF1 in green, LpqE in blue and SOD in grey. C) Cartoon representation of the side view of the supercomplex (top) and a cross-sectional view (bottom). The MK is presented as bright green solid spheres and phospholipid as yellow sticks. In the cross-sectional view at the bottom, the boundaries of CIII, CIV and association factor PRSAF1 are depicted in dashed lines in color (orange for CIII, magenta for CIV and green for PRSAF1). Credit: Science, doi: 10.1126/science.aat8923. More information: Hongri Gong et al. An electron transfer path connects subunits of a mycobacterial respiratory supercomplex, Science (2018). DOI: 10.1126/science.aat8923Eric F. Pettersen et al. UCSF Chimera?A visualization system for exploratory research and analysis, Journal of Computational Chemistry (2004). DOI: 10.1002/jcc.20084 During cryo-EM analysis, the researchers used uranyl acetate (1 percent w/v) for negative staining, using 5 µl of the supercomplex sample at a concentration of 0.05 mg/ml, images were taken on a FEI Tecnai Spirit microscope operating at 120 kV for initial model building. The acquired images were processed using a low-resolution reconstruction of the supercomplex from 53 micrographs of the negative stained sample. For complete reconstruction of the supercomplex, the authors manually selected 7,600 micrographs from 8,200 original micrographs during cryo-EM image processing. All figures in the study were created using PyMOL or UCSF chimera. The composition and structure of CIV. Cartoon representation of the complex IV. The phospholipids are shown as yellow sticks. Credit: Science, doi: 10.1126/science.aat8923 This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. © 2018 Science X Network Typically, chemical energy to synthesize adenosine triphosphate (ATP) and power cellular reactions is extracted during cellular respiration by coupling the oxidation of an energy source (sugars, fatty acids and amino acids) and the reduction of an electron acceptor (oxygen, sulfur, nitrate and sulfate). In aerobic cellular respiration, energy is extracted from electron donors to the terminal acceptor, oxygen, via the electron transport chain (ETC) to create a transmembrane proton gradient known as a proton motive force (PMF) that drives ATP synthesis. The new results now published in Science reveal a direct link for electron transfer between enzymes to represent a new mode of respiratory chain catalysis. Quinones and cytochromes are two types of electron carriers in ETCs used to shuttle electrons to and from large macromolecular structures embedded in the membrane. Four membrane oxidoreductases are involved in the mitochondrial respiratory chain for electron transfer. These include complex I (NADH:ubiquinone oxidoreductase, CI), complex II (succinate:ubiquinone oxidoreductase, CII), complex III (bc1-type ubiquinol:cytochrome c oxidoreductase, bc1-type CIII) and complex IV (aa3-type cytochrome c oxidase, aa3-type CIV). By function, CIII can oxidize ubiquinol to ubiquinone and pass the electrons to soluble cytochrome c. Electrons are then shuttled to CIV, where oxygen is reduced to water. The transmembrane PMF is generated by proton pumping in CI, CIII and CIV. The composition and structure of CIII dimer. The menaquinone/menaquinol is presented as bright green solid spheres and the phospholipids are yellow sticks. Credit: Science, doi: 10.1126/science.aat8923 Journal information: Science During bacterial cell culture experiments, the authors used an M. tuberculosis-like hydrogen peroxide-resistant M. smegmatis mutant strain. The cells were cultured and the membrane isolated as previously described. After cell culture, harvesting and cell lysis, cell membrane pellets were harvested to extract respiratory supercomplexes. The supercomplexes were characterized using optical spectroscopy, mass spectroscopy and 3,3′-diaminobenzidine (DAB) staining. To identify heme groups, selected fractions were analyzed by recording spectra before and after reduction with dithionate as described previously. The purified samples were analyzed using native mass spectroscopy to investigate the architecture and the individual structural components were analyzed using previously established protocols. Citation: Ready for its close-up—a bacterium’s electron transport pathway (2018, November 5) retrieved 18 August 2019 from Explore furtherlast_img read more

first_imgTwo-and-a-half years after he met with a crippling road accident that cut short his career, veteran Malayalam film actor Jagathy Sreekuamar was awarded Rs 5.90 crore by a private insurance company as compensation. The documents regarding the award of compensation was handed over to the wheelchair-bound actor at his residence here by top officials of the insurance company. The actor and his family members had earlier clinched an agreement with the insurance company at an Adalat conducted by the District Legal Services Authority. Jagathy met with a major road accident while travelling in a car at Thenhippalam near Kozhikode in March 2012, when he was on his way to a shooting location at Kodagu in Karnataka. This followed prolonged treatment in hospitals.last_img

first_imgThere were long delays on the M6 motorway in one direction following an accident tonight. Highways England and the Central Motorway Police Group, as well as paramedics from West Midlands Ambulance Service, were called to the Northbound carriageway between Junction 14 (Stafford North) and junction 15 (Hanchurch/Stoke-on-Trent). The carriageway was fully closed from around 6.30pm but one lane is understood to have reopened at around 7.45pm, followed by a second one at around 8.45pm.  The last lane reopened at around 9.35pm. A Highways England spokesman said:  “All lanes have now been opened M6 Northbound J14 Creswell to J15 Hanchurch.Scene has been cleared. Expect delays.” Some motorists reported delays of more an hour. We’ll bring you live updates as we get them on the feed below. Read MoreMotorway police explain why they shut M6 after finding 27 migrants in lorry   Want to tell us about something going on where you live? Let us know – Tweet us  @SOTLive  or message us on  our Facebook page . And if you have pictures to share, tag us on Instagram at  StokeonTrentLive  . 21:38All lanes openAll lanes have now reopened.21:02Vehicles being recoveredInrix are reporting the vehicles are currently being recoverd. The lorry has been recovered and police are on scene awaiting recovery for the car. Affecting traffic to J14.20:49Latest from Inrix One lane closed and queueing traffic due to accident, a lorry and a car involved on M6 Northbound between M6 (Stafford Services) and J15 A500 D Road (Stoke-On-Trent). In the roadworks area. Travel time is 60 minutes.Camera suggest lane three (of three) remains closed, after all traffic being held around 18:45. Police and ambulance services are on scene. 20:47Two lanes now reported openWe’re now getting reports two lanes are open.20:34Two lanes remain closedTwo lanes remain closed on the M6.20:18Traffic now expected to return to normal by 11.30pmTraffic England is now reporting traffic is expected to return to normal by 11.30pm. Again though these times do change a lot.20:12Long queues continueLong delays continue (Image: Highways England)20:00Ambulance making its way to the scene tonightAn ambulance making its way to the scene tonight19:55Ambulance service also at sceneInrix, the traffic data company, also reports West Midlands Ambulance Service are at the scene of the collision. We have no word on if anyone has been hurt at this stage.19:49One lane now openHighways England have confirmed one lane is now open.A spokesman said: “Lane 1 has been opened to allow traffic through M6 Northbound J14 Creswell to J15 Hanchurch. Traffic Officers and Central Motoeway Police are on Scene. Expect delays.”19:49Incident expected to clear by 10pmTraffic England are now reporting the road is expected to clear by 10pm. It is worth noting these times often change considerably.19:36Delays of more than an hour now reportedSome motorists are saying they have not moved for more than an hour19:30What we know so farThe M6 Northbound is blocked between J14 (Stafford) and J15 (Stoke-on-Trent)A number of vehicles are involved in the accident, with Inrix reporting a car and a lorryMotorists are reporting not moving for more than 40 minutes19:28Still no sign of movementMotorists are now reporting they have been stuck in traffic for around 40 minutes.19:17Inrix reports lorry and car involvedInrix, the traffic data company, report the accident involves a lorry and a car19:1530 minute delays reportedThere are currently 30 minute delays reported.19:14One of the vehicles involvedOne of the vehicles involved19:09Police making their way to the scenePolice making their way to the scene19:05Number of vehicles believed to be involvedA number of vehicles, including a lorry, are reported to be involved19:04Photo from the sceneTraffic queueing on the M6 this evening19:04Another photo from the sceneAnother photo 18:56Location of the accidentThe affected stretch of carriageway tonight (Image: Inrix)18:55Latest from InrixStationary traffic due to all traffic being temporarily held and accident on M6 Northbound between M6 (Stafford Services) and J15 A500 D Road (Stoke-On-Trent). In the roadworks area.Just before 18:45 all traffic was held to assist with an accident.last_img read more

first_imgNovember 6, 2003BRIDGE POUR: A second bridge is poured at the construction site behind the East Crescent.[Photo: aa & text: kh] As part of a synergy, workshoppers and members of the agriculture crew helped with the concrete work.[Photo: aa & text: kh] Each batch of concrete is mixed on site and carried by wheel barrel to the bridge frame.[Photo: aa & text: kh] With the help of hands from many departments, the process went smoothly and quickly.[Photo: aa & text: kh]last_img