first_imgNote, we weren’t looking for page views, necessarily, but we got them. Who knew FOLIO:’s Web site would become a forum for debating abortion rights issues? Since posting this story Monday (“Pro-Life Groups Outraged Over Vogue Photo Spread”) about how a photoshoot for a Vogue column on partial-birth abortions has angered religious and pro-life groups—who say it makes the controversial procedure appear fashionable—thousands of people have stopped by to read it, and many have posted passionate comments rationalizing all sides of the issue.Our story was even picked up by Page Six today, which may or may not be a first for FOLIO:. last_img

first_img Facebook Ticketmaster’s Verified Fan Blocks Ticket Bots ticketmasters-verified-fan-successfully-blocks-ticket-bots Ticketmaster’s Verified Fan Successfully Blocks Ticket Bots Twitter Email News NETWORK ERRORCannot Contact ServerRELOAD YOUR SCREEN OR TRY SELECTING A DIFFERENT VIDEO May 15, 2017 – 1:50 am Watch: Adele wins Album Of The Year for ’25’ The program to block ticket bots and brokers from buying tickets shows a 90 percent success rateRenée FabianGRAMMYs Sep 8, 2017 – 6:12 pm The battle against ticket bots has plagued the ticket sales industry, and especially the live music business. But Live Nation’s Ticketmaster seems to be making strides with their Verified Fan program. Verified Fan separates actual music fans from bots and scalpers by allowing fans to pre-register to order tickets. Once the system verifies an actual human is looking to purchase tickets as opposed to a bot, the fan receives a pre-registration code that “unlocks the opportunity to purchase tickets.”According to Live Nation CEO Michael Rapino, they’ve had a 90 percent success rate so far, which is crucial for fans who want to land a ticket to see their favorite artists. The demand is always higher than the number of tickets available. For example, more than 10 million Adele fans attempted to buy tickets for her 2016 tour, but only 400,000 seats were available.As demand for live shows continues to increase, blocking bots is good for both fans and business alike.”There’s probably not been an industry with compounded growth as consistent as the live music business,” Rapino said. “Live concerts continually ranked in the top three things that a consumer wants to attend every year as their escape.”Apple Secures Deal With Warner Music Grouplast_img read more

first_imgrangamatiA member of United People’s Democratic Front (UPDF) was hacked to death by some miscreants at his residence in Bagaichhari upazila on early Sunday.The deceased was identified as Natun Moni Chakma, 42, of Balukhali village.United People’s Democratic Front or UPDF is a regional political party representing ethnic people that opposed the Chittagong Hill Tracts peace treaty signed in 1997 to bring an end to insurgency.Local people said some miscreants hacked dead Natun Moni with sharp weapons when he was sleeping at his yard.On information, the police recovered the body in the morning and sent to Khagrachhari Hospital morgue for autopsy.However, the police could not immediately ascertain who the mastermind and assailants are, according to officer-in-charge of Baghaichhari police station Amir Hossain.last_img read more

first_img Share wikimedia commonsU.S. Southern Command, Commander Gen. John F. Kelly briefs the media on the current state of USSOUTHCOM affairs, March 20, 2013. (DoD photo by Mass Communication Specialist 1st Class Chad J. McNeeley/Released)“Dreamers” protected by an Obama-era program that President Donald Trump has said will end on March 5 are not top targets for deportation as long as they lack criminal records, the White House chief of staff said Tuesday.John Kelly also dismissed the idea of a short-term extension of the Deferred Action for Childhood Arrivals program beyond March 5 to give the administration and Congress more time to work out compromise legislation.“What makes them act is pressure,” he said of Congress.Kelly’s remarks, to a small group of reporters at the Capitol, came as lawmakers have deadlocked in an effort to reach a bipartisan deal on protecting from deportation recipients of the program, called DACA. Barring a last-minute agreement — which seems unlikely — Senate Majority Leader Mitch McConnell, R-Ky., has said his chamber will begin considering the issue, a debate that GOP leaders expect to start next week.A federal judge has indefinitely blocked Trump from terminating DACA’s protections for the so-called Dreamers, who were brought to the U.S. as children and are living here illegally. The program shields them from deportation and gives them the right to hold jobs.Many lawmakers are uneasy about what might happen to the Dreamers after March 5, and Democrats — and Trump himself — are using that uncertainty as leverage to help force a deal. Kelly’s remarks seemed aimed at easing worries that major deportations of Dreamers could begin right away.He described a lengthy legal process that often takes years that immigrants in the U.S. illegally face before they are deported. He said an immigrant here illegally who doesn’t commit crimes “never comes onto anyone’s scope.”He added, “They are not a priority for deportation.”Trump has proposed creating a pathway to citizenship for up to 1.8 million immigrants covered by DACA who might potentially qualify for its protections.In exchange, Trump wants $25 billion for border security, including money to build his much-touted wall along the U.S.-Mexico border. He also wants to curb legal immigration, restricting the relatives that legal immigrants could sponsor for citizenship and ending a lottery that distributes visas to people from diverse places like Africa.Democrats strongly oppose limiting legal immigration, and conservatives are against giving citizenship to DACA recipients. Trump’s bill has gotten little traction in Congress.Kelly said he doubted Trump would act unilaterally to extend DACA beyond March 5. He cited the administration’s argument that former President Barack Obama exceeded his constitutional authority in initially creating it.He also ruled out asking lawmakers to approve a short-term extension of the program. And he said he opposed one idea mentioned in the Capitol as a possible fallback should talks on a broader bipartisan compromise fail — a one-year extension of DACA in exchange for a year’s worth of money for border security, including for building Trump’s wall.“I would certainly advise against it,” Kelly said. “I would advise against it, I’m not the president.”Trump said last September that he was ending the DACA program, complaining that Obama had overstepped his legal authority in creating it.But while his 2016 election campaign was laced with anti-immigrant remarks, he’s expressed sympathy for the Dreamers and he gave Congress until March 5 to pass legislation protecting them.last_img read more

first_imgIn the prokaryotic respiratory chain, the situation is more complicated. A complete pathway of electron flow has not yet been determined in the cell type due to its complexity. It is therefore necessary to understand the complete structure of a “supercomplex” involved during bacterial electron transfer to assist the goal. In the study, the researchers extracted and purified the complex from M. smegmatis to visualize the architecture using cryo-electron microscopy (cryo-EM) at a resolution of 3.5 Å. The structure provided crucial insights into the mechanism of direct electron transfer within a respiratory supercomplex. The dimensions of the supercomplex were in the range of 200 x 70 x 120 Å, in a symmetrized linear architecture completely different from previously reported respiratory supercomplexes. By composition, the linear dimeric CIV1-CIII2-CIV1 was arranged such that individual CIVs flanked the central CIII dimer on either side. The information revealed a direct link between enzymes during electron transfer, representing a new mode of respiratory chain catalysis. The detailed structural findings have potential to assist with antimycobacterial drug discovery efforts. The architecture of the supercomplex in its entirety: The cartoon representation shows the menaquinone/menaquinol (MK) in bright green colored solid spheres and phospholipids as yellow sticks. Credit: Science, doi: 10.1126/science.aat8923 The authors revealed the cryo-EM structure of a CIII-CIV respiratory supercomplex of the M. smegmatis bacteria. The intra-complex electron transfer pathway ranged from quinol oxidation in CIII to oxygen reduction in CIV. The results showed a new mechanism for bifurcating electron transfer to ensure completion of the Q cycle (the net movement of protons across a lipid bilayer) for energy transduction. The association of a superoxide dismutase in the architecture of the system can protect against oxidative damage by reactive oxygen species (ROS). The architecture of quinone binding sites also provided a framework for future studies in structure-based antimicrobial drug discovery. Study shows how bacteria guide electron flow for efficient energy generation In a recent study conducted by Hongri Gong and colleagues, a respiratory supercomplex was isolated from the bacterium Mycobacterium smegmatis, and its structure was visualized at a resolution of 3.5 Å using cryo-electron microscopy (cryo-EM). The bacterium is a close relative to M. tuberculosis and a popular model used to study many other bacterial species. The detailed structure revealed how electrons were transferred in the cell in a process hitherto unseen. Respiration in Actinomycetes and overall architecture of the Mycobacterial respiratory machine CIII2CIV2SOD2. A) The respiratory electron transfer chain in Actinomycetes (left) and the 5 major prokaryotic cytochrome c pathway variants with the organization schemes in representative organisms (right). The M. smegmas cytochrome c pathway has its cytochrome c fused with complex III to form a bcc-type complex III that interacts with the aa3-type complex IV to assemble into a CIII-CIV supercomplex. MK = menaquinone/menaquinol. B) The entire architecture of the bcc-aa3 type respiratory CIII-CIV Supercomplex from M. smegmatis. The cryo-EM map of the supercomplex shows a linear 2-fold dimerized form of CIV1-CIII2-CIV1 with dimensions 200 x 70 x 120 Å. CIII is seen in orange, CIV is magenta and the association factors PRSAF1 in green, LpqE in blue and SOD in grey. C) Cartoon representation of the side view of the supercomplex (top) and a cross-sectional view (bottom). The MK is presented as bright green solid spheres and phospholipid as yellow sticks. In the cross-sectional view at the bottom, the boundaries of CIII, CIV and association factor PRSAF1 are depicted in dashed lines in color (orange for CIII, magenta for CIV and green for PRSAF1). Credit: Science, doi: 10.1126/science.aat8923. More information: Hongri Gong et al. An electron transfer path connects subunits of a mycobacterial respiratory supercomplex, Science (2018). DOI: 10.1126/science.aat8923Eric F. Pettersen et al. UCSF Chimera?A visualization system for exploratory research and analysis, Journal of Computational Chemistry (2004). DOI: 10.1002/jcc.20084 During cryo-EM analysis, the researchers used uranyl acetate (1 percent w/v) for negative staining, using 5 µl of the supercomplex sample at a concentration of 0.05 mg/ml, images were taken on a FEI Tecnai Spirit microscope operating at 120 kV for initial model building. The acquired images were processed using a low-resolution reconstruction of the supercomplex from 53 micrographs of the negative stained sample. For complete reconstruction of the supercomplex, the authors manually selected 7,600 micrographs from 8,200 original micrographs during cryo-EM image processing. All figures in the study were created using PyMOL or UCSF chimera. The composition and structure of CIV. Cartoon representation of the complex IV. The phospholipids are shown as yellow sticks. Credit: Science, doi: 10.1126/science.aat8923 This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. © 2018 Science X Network Typically, chemical energy to synthesize adenosine triphosphate (ATP) and power cellular reactions is extracted during cellular respiration by coupling the oxidation of an energy source (sugars, fatty acids and amino acids) and the reduction of an electron acceptor (oxygen, sulfur, nitrate and sulfate). In aerobic cellular respiration, energy is extracted from electron donors to the terminal acceptor, oxygen, via the electron transport chain (ETC) to create a transmembrane proton gradient known as a proton motive force (PMF) that drives ATP synthesis. The new results now published in Science reveal a direct link for electron transfer between enzymes to represent a new mode of respiratory chain catalysis. Quinones and cytochromes are two types of electron carriers in ETCs used to shuttle electrons to and from large macromolecular structures embedded in the membrane. Four membrane oxidoreductases are involved in the mitochondrial respiratory chain for electron transfer. These include complex I (NADH:ubiquinone oxidoreductase, CI), complex II (succinate:ubiquinone oxidoreductase, CII), complex III (bc1-type ubiquinol:cytochrome c oxidoreductase, bc1-type CIII) and complex IV (aa3-type cytochrome c oxidase, aa3-type CIV). By function, CIII can oxidize ubiquinol to ubiquinone and pass the electrons to soluble cytochrome c. Electrons are then shuttled to CIV, where oxygen is reduced to water. The transmembrane PMF is generated by proton pumping in CI, CIII and CIV. The composition and structure of CIII dimer. The menaquinone/menaquinol is presented as bright green solid spheres and the phospholipids are yellow sticks. Credit: Science, doi: 10.1126/science.aat8923 Journal information: Science During bacterial cell culture experiments, the authors used an M. tuberculosis-like hydrogen peroxide-resistant M. smegmatis mutant strain. The cells were cultured and the membrane isolated as previously described. After cell culture, harvesting and cell lysis, cell membrane pellets were harvested to extract respiratory supercomplexes. The supercomplexes were characterized using optical spectroscopy, mass spectroscopy and 3,3′-diaminobenzidine (DAB) staining. To identify heme groups, selected fractions were analyzed by recording spectra before and after reduction with dithionate as described previously. The purified samples were analyzed using native mass spectroscopy to investigate the architecture and the individual structural components were analyzed using previously established protocols. Citation: Ready for its close-up—a bacterium’s electron transport pathway (2018, November 5) retrieved 18 August 2019 from https://phys.org/news/2018-11-ready-close-upa-bacterium-electron-pathway.html Explore furtherlast_img read more

first_imgKolkata: Members of the Matua community staged demonstrations at different parts of the state, protesting against the exclusion of the names of 40 lakh Bengalis in the final draft of Assam’s National Register of Citizens (NRC).It may be mentioned that Chief Minister Mamata Banerjee had stated that with exclusion of their names, the people have turned refugees in their own country and it is the outcome of BJP’s “vote politics”.Stating that the “people are being isolated”, the Chief Minister had said: “BJP’s divide and rule policy to isolate people has led to the situation.” Also Read – Rain batters Kolkata, cripples normal lifeThe Chief Minister, who is in Delhi at present, criticised the government at the Centre over Assam’s NRC issue.The protest started at around 8 am and continued till noon. People from the community carried out agitation mainly in North 24-Parganas and Nadia districts. They also put up a symbolic railway blockade at Panihati Railway Station.With the protest, there was a disruption in train services since morning at different stations of Sealdah Division. Also Read – Speeding Jaguar crashes into Mercedes car in Kolkata, 2 pedestrians killedThere were obstructions at various stations of the Sealdah-Ranaghat Main Line, Sealdah-Bangaon, Sealdah-Dankuni and Barasat Hansnabad sections.There were obstructions at Sandalia, New Barrackpore, Gobardanga, Dum Dum, Belghoria, Ichhapur, Kanchrapara, Chakdaha, Halisahar, Bira, Naihati, Titagarh, Palta, Baranagar, Sodepur, Dankuni, Machlandapur, Habra, Ashoknagar, Duttapukur, Bamangachi, Barrackpore, Badkulla,Bongaon, Ranaghat, Akaipur, and Thakurnagar. The train services resumed at around 12 noon. There was cancellation of 51 local trains of Sealdah Division.People from the Matua community also protested against the move by putting up a roadblock at Bagnan in Howrah on National Highway 6, demanding immediate steps so that people do not turn refugees in their own country.There were long queues of vehicles on the national highway. It took some time to normalise the traffic movement after the demonstration was withdrawn.Commuters were greatly inconvenienced as train services in the main section were badly hit. The demonstration was lifted and train services became normal from noon.last_img read more