first_imgSevilla boss Pablo Machin is keen on securing a place in the knockout stages of the Europa League suggesting they will give it their all in the last group tieThe La Liga side who are the record holders of the competition with five titles in the last 12 years – lost 1-0 at Standard Liege on Thursday night, meaning they must beat FC Krasnodar in their last game to qualify.“We have failed again and when this happens in Europe, your position becomes complicated,” Machin told reporters, as cited by Football Espana via the club media.“We were not allowed to play the game we wanted to play and they hurt us on the break, then when Pablo (Sarabia) was sent-off we struggled, that moment killed us.Top 5 La Liga players to watch for next weekend’s activity Tomás Pavel Ibarra Meda – September 11, 2019 With the international activity cooling down for the next month, we go back to the Top 5 players to watch in La Liga next…“We now know that to progress is not going to be a stroll in the park and that we will have to fight tooth and nail.“But we are at home in front of our fans and they will push us on to qualify in what will be a difficult match.“This has been a demanding group.”last_img read more

first_img Share your voice Random Post a comment 13 Photos Bill Gates Bill Gates has some suggestions for your 2019 summer reading list. Bill Gates/Gates Notes Summer vacation is almost here in the northern hemisphere. Woo-hoo! You’re heading to the beach. You could pack a romance novel or a lightweight spy caper. Or you could pack the five books Microsoft founder Bill Gates recommends you read. Get ready to get heavy.Gates’ summer reading list is an annual tradition. He released the latest version on his Gates Notes blog on Monday, giving you plenty of time to hit your local bookstore for some thought-provoking reading to pack with your sunscreen and bathing suit.”I’ve recently found myself drawn to books about upheaval (that’s even the title of the one of them) — whether it’s the Soviet Union right after the Bolshevik revolution, the United States during times of war, or a global reevaluation of our economic system,” Gates wrote, saying most of these books deal with the idea of disruption. 0 UpheavalJared Diamond’s Upheaval delves into “how nations managed existential challenges like civil war, foreign threats and general malaise” through a group of case studies. Gates acknowledged this might sound like a bummer, but said he left the book feeling optimistic about our ability to solve problems.Nine PintsIf you made it through all the bloodshed of Game of Thrones, then chances are good you can handle Nine Pints by Rose George. The subtitle clues you into the subject: “A Journey Through the Money, Medicine, and Mysteries of Blood.” “It’s filled with super-interesting facts that will leave you with a new appreciation for blood,” Gates said.A Gentleman in MoscowNow we detour into the realm of fiction with Amor Towles’ A Gentleman in Moscow. This bestselling novel follows the rich Count Alexander Rostov who is living under house arrest in a hotel in Moscow in the 1920s. The book spans several decades. Gates called it “fun, clever, and surprisingly upbeat.” Presidents of WarMichael Beschloss’ Presidents of War tackles the complicated history of US presidents and the conflicts the country became involved in, including the War of 1812 and the Vietnam War. “Beschloss’s broad scope lets you draw important cross-cutting lessons about presidential leadership,” said Gates.The Future of CapitalismGates said he doesn’t agree with everything in Paul Collier’s The Future of Capitalism, but that the author’s “background as a development economist gives him a smart perspective on where capitalism is headed.” Gates recommended this as a good analysis of the problems around capitalism today.While these five titles make up the official summer reading list, Gates also threw in a plug for his wife Melinda Gates’ The Moment of Lift, a book about the women who have inspired her. “I know I’m biased, but it’s one of the best books I’ve read so far this year,” he said. These books should keep you busy until the end of 2019 when we can expect Gates’ annual list of his favorite books of the year. 1:08 Tags How to check out digital library books Inside Microsoft’s HoloLens development lab Now playing: Watch this:last_img read more

first_imgThe country’s development prospects will be bleak if the projected growth of population is not controlled immediately, a Prothom Alo roundtable was told on Monday.Already, experts said, Bangladesh is facing a variety of challenges, including employment and shortage of land due to the huge size of population on a small landmass.They added, Bangladesh’s earlier success in population growth control has been stagnated in recent times, and emphasised the urgent need for revitalising the past family planning programmes, resuming visits to door to door by field workers.”Our dream to become a middle income country will be jeoparadised if the population is not managed,” Hossain Zullur, executive chairman of Power and Participation Research Centre (PPRC), said presenting the keynote at the workshop.”The success we have achieved has become stagnant as we have relaxed our past strategy to visit door to door for campaign to create awareness about the family planning,” added the economist, also a former caretaker government adviser.The roundtable titled ‘family planning: empowerment of people and development of nation’, was organised at the Prothom Alo office with support from the United Nations Population Fund (UNFPA). The discussion was moderated by Prothom Alo associate editor Abdul Qayyum.According to the 2011 population census, the country’s recent population is more than 160.30 million and it is projected to be 280 million by 2061, it was mentioned in the keynote. Technical officer of family planning of UNFPA Abu Sayed Hasan authored it.On the current situation, Hossain Zillur Rahman said the country is facing the crisis of employment opportunity.Around 2.2 million people are adding to the workforce, but 700,000 people are being provided with jobs domestically and another 500,000 people abroad, he pointed out.Hossain Zillur said the land cannot be found for implementing the projects.In 20 years between 1994 and 2014, the proportion of women visited by family planning field workers has been almost halved.However, director general of family planning Kazi Mustafa Sarwar said over 400 physicians have been appointed to strengthen the family planning activities in the field level.Speaking as the chief guest, health minister Mohammad Naism said the population growth in Sylhet and Chittagong is very high as being conservative, the people there do not want to receive family planning methods.He urged Prothom Alo authorities to arrange seminars and discussions at slum and rural areas and he will attend those, rather arranging it in drawing rooms and conference halls.Law maker Fazilatunnesa Bappy said family planning activities are very urgently needed to implement sustainable development goals (SDG).She said men also have to be included in the programmes.Chairman of Population Science Department at Dhaka University professor Aminul Haque said the family planning is not possible only by controlling birth. Overall matters including health and education have to be included in the planning.Actress Peya Jannatul said the people have to be made aware through drama.Youth representative Tasneem Jara said women have to be empowered by providing them with knowledge about contraception.Youths have to be utilised as they want to work on population issues, she added.While moderating, Abdul Qayyum said the additional population is number one problem.”We have not achieved progress to our desired level. We have to increase investment,” he said.Line director at the directorate of family planning Moinuddin Ahmed, chief of party USAID-DFID NGO Health Service Delivery Project Halida Hanum Akhtar, Associate of Population Council Sharif Mohammed Ismail Hossain, Ipas country manager Sayed Rubayet, Islamic Foundation Director Khijir Hayat Khan and genereral manager of SMC Toslim Uddin Khan, among others, also spoke.last_img read more

first_imgNeo JMB leader Abu Sayeed alias Karim alias Shyamal was produced before the journalists at the office of Detective Branch of police in Bogra on Saturday noon. Photo: Sohel RanaDetective Branch of police overnight arrested most wanted JMB leader Abu Sayeed, a death row convict, from Omarpur area of Nandigram upazila in the district.Sayeed alias Karim alias Shyamal was the main accused of Khagragar bomb blast in Burdwan in the West Bengal state of India.A team of detectives of Police Headquarters and detective branch of police in Bogra conducted a drive at the Nandigram upazila around 1 am last night and arrested him and recovered firearms, ammunitions and a motorcycle.He was the southern wing chief of banned militant outfit Jama’atul Mujahideen Bangladesh (JMB), superintendant of police of Bogra Md Asaduzzaman told a press conference Saturday afternoon.Police sources said Sayeed joined JMB in 2002. In 2005, he was awarded death penalty in a case of launching bomb explosion in Naogaon. He fled to India in 2007.He got married to Khadiza, a daughter of militant outfit member Yadul in Murshidabad district and used to operate militant activities there.In 2014, Sayeed was accused of Burdwan blast. A case was lodged against him there.Following the Burdwan blast, National Investigation Agency (NIA) in Kolkata declared a reward of one million Indian rupees for credible information on his whereabouts. In 2015, he fled to Bangladesh.last_img read more

first_imgIn the prokaryotic respiratory chain, the situation is more complicated. A complete pathway of electron flow has not yet been determined in the cell type due to its complexity. It is therefore necessary to understand the complete structure of a “supercomplex” involved during bacterial electron transfer to assist the goal. In the study, the researchers extracted and purified the complex from M. smegmatis to visualize the architecture using cryo-electron microscopy (cryo-EM) at a resolution of 3.5 Å. The structure provided crucial insights into the mechanism of direct electron transfer within a respiratory supercomplex. The dimensions of the supercomplex were in the range of 200 x 70 x 120 Å, in a symmetrized linear architecture completely different from previously reported respiratory supercomplexes. By composition, the linear dimeric CIV1-CIII2-CIV1 was arranged such that individual CIVs flanked the central CIII dimer on either side. The information revealed a direct link between enzymes during electron transfer, representing a new mode of respiratory chain catalysis. The detailed structural findings have potential to assist with antimycobacterial drug discovery efforts. The architecture of the supercomplex in its entirety: The cartoon representation shows the menaquinone/menaquinol (MK) in bright green colored solid spheres and phospholipids as yellow sticks. Credit: Science, doi: 10.1126/science.aat8923 The authors revealed the cryo-EM structure of a CIII-CIV respiratory supercomplex of the M. smegmatis bacteria. The intra-complex electron transfer pathway ranged from quinol oxidation in CIII to oxygen reduction in CIV. The results showed a new mechanism for bifurcating electron transfer to ensure completion of the Q cycle (the net movement of protons across a lipid bilayer) for energy transduction. The association of a superoxide dismutase in the architecture of the system can protect against oxidative damage by reactive oxygen species (ROS). The architecture of quinone binding sites also provided a framework for future studies in structure-based antimicrobial drug discovery. Study shows how bacteria guide electron flow for efficient energy generation In a recent study conducted by Hongri Gong and colleagues, a respiratory supercomplex was isolated from the bacterium Mycobacterium smegmatis, and its structure was visualized at a resolution of 3.5 Å using cryo-electron microscopy (cryo-EM). The bacterium is a close relative to M. tuberculosis and a popular model used to study many other bacterial species. The detailed structure revealed how electrons were transferred in the cell in a process hitherto unseen. Respiration in Actinomycetes and overall architecture of the Mycobacterial respiratory machine CIII2CIV2SOD2. A) The respiratory electron transfer chain in Actinomycetes (left) and the 5 major prokaryotic cytochrome c pathway variants with the organization schemes in representative organisms (right). The M. smegmas cytochrome c pathway has its cytochrome c fused with complex III to form a bcc-type complex III that interacts with the aa3-type complex IV to assemble into a CIII-CIV supercomplex. MK = menaquinone/menaquinol. B) The entire architecture of the bcc-aa3 type respiratory CIII-CIV Supercomplex from M. smegmatis. The cryo-EM map of the supercomplex shows a linear 2-fold dimerized form of CIV1-CIII2-CIV1 with dimensions 200 x 70 x 120 Å. CIII is seen in orange, CIV is magenta and the association factors PRSAF1 in green, LpqE in blue and SOD in grey. C) Cartoon representation of the side view of the supercomplex (top) and a cross-sectional view (bottom). The MK is presented as bright green solid spheres and phospholipid as yellow sticks. In the cross-sectional view at the bottom, the boundaries of CIII, CIV and association factor PRSAF1 are depicted in dashed lines in color (orange for CIII, magenta for CIV and green for PRSAF1). Credit: Science, doi: 10.1126/science.aat8923. More information: Hongri Gong et al. An electron transfer path connects subunits of a mycobacterial respiratory supercomplex, Science (2018). DOI: 10.1126/science.aat8923Eric F. Pettersen et al. UCSF Chimera?A visualization system for exploratory research and analysis, Journal of Computational Chemistry (2004). DOI: 10.1002/jcc.20084 During cryo-EM analysis, the researchers used uranyl acetate (1 percent w/v) for negative staining, using 5 µl of the supercomplex sample at a concentration of 0.05 mg/ml, images were taken on a FEI Tecnai Spirit microscope operating at 120 kV for initial model building. The acquired images were processed using a low-resolution reconstruction of the supercomplex from 53 micrographs of the negative stained sample. For complete reconstruction of the supercomplex, the authors manually selected 7,600 micrographs from 8,200 original micrographs during cryo-EM image processing. All figures in the study were created using PyMOL or UCSF chimera. The composition and structure of CIV. Cartoon representation of the complex IV. The phospholipids are shown as yellow sticks. Credit: Science, doi: 10.1126/science.aat8923 This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. © 2018 Science X Network Typically, chemical energy to synthesize adenosine triphosphate (ATP) and power cellular reactions is extracted during cellular respiration by coupling the oxidation of an energy source (sugars, fatty acids and amino acids) and the reduction of an electron acceptor (oxygen, sulfur, nitrate and sulfate). In aerobic cellular respiration, energy is extracted from electron donors to the terminal acceptor, oxygen, via the electron transport chain (ETC) to create a transmembrane proton gradient known as a proton motive force (PMF) that drives ATP synthesis. The new results now published in Science reveal a direct link for electron transfer between enzymes to represent a new mode of respiratory chain catalysis. Quinones and cytochromes are two types of electron carriers in ETCs used to shuttle electrons to and from large macromolecular structures embedded in the membrane. Four membrane oxidoreductases are involved in the mitochondrial respiratory chain for electron transfer. These include complex I (NADH:ubiquinone oxidoreductase, CI), complex II (succinate:ubiquinone oxidoreductase, CII), complex III (bc1-type ubiquinol:cytochrome c oxidoreductase, bc1-type CIII) and complex IV (aa3-type cytochrome c oxidase, aa3-type CIV). By function, CIII can oxidize ubiquinol to ubiquinone and pass the electrons to soluble cytochrome c. Electrons are then shuttled to CIV, where oxygen is reduced to water. The transmembrane PMF is generated by proton pumping in CI, CIII and CIV. The composition and structure of CIII dimer. The menaquinone/menaquinol is presented as bright green solid spheres and the phospholipids are yellow sticks. Credit: Science, doi: 10.1126/science.aat8923 Journal information: Science During bacterial cell culture experiments, the authors used an M. tuberculosis-like hydrogen peroxide-resistant M. smegmatis mutant strain. The cells were cultured and the membrane isolated as previously described. After cell culture, harvesting and cell lysis, cell membrane pellets were harvested to extract respiratory supercomplexes. The supercomplexes were characterized using optical spectroscopy, mass spectroscopy and 3,3′-diaminobenzidine (DAB) staining. To identify heme groups, selected fractions were analyzed by recording spectra before and after reduction with dithionate as described previously. The purified samples were analyzed using native mass spectroscopy to investigate the architecture and the individual structural components were analyzed using previously established protocols. Citation: Ready for its close-up—a bacterium’s electron transport pathway (2018, November 5) retrieved 18 August 2019 from https://phys.org/news/2018-11-ready-close-upa-bacterium-electron-pathway.html Explore furtherlast_img read more

first_imgKolkata: The Election Commission has instructed the District Election Officer (DEO) of West Burdwan to register two separate FIRs against Babul Supriyo. The Commission issued instructions after Supriyo had allegedly snatched a video camera from an official deputed by the Commission who was capturing videos as part of the surveillance that is being carried out by the Election Commission.The district official has been directed to file an FIR for the incident. Also Read – Rs 13,000 crore investment to provide 2 lakh jobs: MamataAnother FIR would be registered against him as he failed to submit any convincing reply as to why he released and played the controversial theme song of his party after being banned by the Commission. Meanwhile, the Commission has sought a report from the district administrative officials in West Burdwan on the incident in which BJP activists led by Asansol sitting MP Babul Supriyo stormed into the room of an Officer-in-Charge at the local police station and threatened him. Also Read – Lightning kills 8, injures 16 in stateA complaint was lodged with the Election Commission following which the DEO has been asked to submit a report on the entire incident that unfolded in Asansol. Supriyo had triggered controversy on a number of occasions since the notification of the Lok Sabha elections. He was earlier show-caused by the Election Commission after he released a theme song for his party. In the most recent incident, Supriyo has been accused of threatening an on-duty police officer at Kulti police station. The BJP leaders in the district claimed that some cops from the local police station ransacked the house of a BJP leader on Saturday evening. Women members of his family were also heckled by the policemen, alleged BJP. Many BJP leaders including women members of the party gheraoed the police post on Saturday night. The police officer told Supriyo that he respects him and urged him to talk properly. But Supriyo allegedly threatened him and said he would not seek any respect from the officer.last_img read more