first_imgReal Madrid forward Marco Asensio, revealed the main reasons for his recent dip in form for his club and gave Solari the thumbs up as the new manager.The new season for young forward Marco Asensio didn’t start as he expected, a player who hasn’t been able to cover the full expectations that were formed around him during his first couple of seasons with Real Madrid.Today with Santiago Solari as the new manager, he is still one of the players who is not considered within the group of footballers who play on a regular basis and has become one of the biggest disappointments for this season.When the new season was about to start, manager Julen Lopetegui decided that he didn’t want to bring any new transfers precisely because he didn’t want to slow the youngster’s progression as a player.Asensio hasn’t been able to respond to this level of trust from Lopetegui, to the point where Solari is not even considering him as an essential part of the squad like he was when Zidane was still around.During the first few months, the player had already stated that he knew more ambition was required from him and he vowed to fight for a secured spot in the squad’s starting eleven.Y @marcoasensio10… ¡Generosidad sin límite! #UnaNuevaIlusión pic.twitter.com/eYpTXJh1K6— Selección Española de Fútbol (@SeFutbol) November 12, 2018Asensio was recently quoted by Diario AS during a television interview to a show called ‘Vamos’, where he gave some details about his lack of participation with the squad this season.“When things are going well, people pin you as the best player in the squad. When things are not going well, they pin you as the worst. In the end, there are some critics who are not fair, but we already know how journalism works in this country,” started Asensio.“I’m obviously generalizing, but I do find some criticisms to be hurtful towards me. Since I don’t really deem it important and I worry about my own performance, it doesn’t really affect me.”“Since Solari arrived things have improved, the results have been on our side. This is football. Solari is our manager now, we would die for any manager who is with us. We all need to be strong as we’ve always been, this will take us back to the winning path.”David Villa, FC BarcelonaTop 10 players who played for both Barcelona and Valencia Boro Tanchev – September 14, 2019 Time to talk about the best players who represented both Barcelona and Valencia, prior to their La Liga encounter at Camp Nou this evening.“I certainly don’t see this episode as a moment of crisis. But I will also tell you that I’m not the one who has to pull the team up inside the club, there are other players who have played in this squad for longer and are more experienced.”“These players have a greater status than mine and they are the ones who need to carry the team. I did play for a few matches with the last manager, but now I don’t play as much as I’d like to but the final assessments will be made by the end of the season,” he concluded.+3. Seguimos trabajando y creciendo juntos. / + 3. We keep on working and growing together. 💪🏽 #RMLiga pic.twitter.com/Yw8F6at2KQ— Marco Asensio (@marcoasensio10) November 3, 2018Asensio is not the only one who has lost the complete confidence from the new manager, both he and Isco Alarcon are the two players who haven’t played a single minute under the Argentine and still have to prove they are worth for him.In Isco’s case, he played pretty much all the matches he could under Julen Lopetegui when he was fit, but he hasn’t played a single minute under Santiago Solari and this is starting to get worrisome for him.As far as Asensio is concerned, this player is going through the same over hyping that many young stars go through and this time is exactly the one in which he needs to be more careful.The press is the one to blame for this situation, because they are the ones who started making early comparisons that went to the kid’s head and reality has slapped him right in the face.Good training 👍🏽⚽ pic.twitter.com/XQlGrcMXDJ— Marco Asensio (@marcoasensio10) November 9, 2018What do you think Marco Asensio should do, stay and fight for a spot in Real Madrid or leave? Please share your opinion in the comment section down below.last_img read more

first_imgWILMINGTON, MA — Below are some of the newest job openings in Wilmington:Full-Time Early Education & Child Care Teacher at Little SproutsFull-Time National Accounts Ecommerce Specialist at UniFirstFull-Time/Part-Time Cashier at Panera BreadFull-Time/Part-Time Food Services Associate at Panera BreadFull-Time Comfort Advisor at Super Service Today, Inc.Temporary Full-TIme Nanny For 2 Wilmington ChildrenFull-Time Order Checker at Arrow Paper Co.Part-Time Package Handler at FedEx GroundFull-Time Realtor at ERA Key Realty ServicesFull-Time Director of Quality Engineering at MKS Instruments(NOTE: Wilmington businesses — Feel free to send me your job postings at wilmingtonapple@gmail.com.)Like Wilmington Apple on Facebook. Follow Wilmington Apple on Twitter. Follow Wilmington Apple on Instagram. Subscribe to Wilmington Apple’s daily email newsletter HERE. Got a comment, question, photo, press release, or news tip? Email wilmingtonapple@gmail.com.Share this:TwitterFacebookLike this:Like Loading… RelatedNOW HIRING: 10 New Job Openings In WilmingtonIn “Business”NOW HIRING: 10 New Job Openings In WilmingtonIn “Business”NOW HIRING: 10 Job Openings In WilmingtonIn “Business”last_img read more

first_imgStatue of Supreme Court Chief Justice Roger Brooke Taney at the Maryland State House. Feb. 23, 2016. (Capital News Service photo by Connor Glowacki.)ANNAPOLIS — Legislation moving through Annapolis could put a statue of Supreme Court Chief Justice Roger Brooke Taney, a Maryland native known for writing the pro-slavery Dred Scott opinion, in jeopardy.Monuments from the Civil War with ties to slavery and the Confederacy have been an increasingly controversial matter throughout Maryland in the past year. Over 20 Democratic delegates are co-sponsoring a House bill that, if passed, would remove and destroy the statue. The bill is set to be introduced to the Maryland House Health and Government Operations committee on Wednesday and has a companion bill in the state Senate, set for a hearing March 4.One of the House bill’s sponsors, Delegate Benjamin Brooks, D-Baltimore County, said that growing up in South Carolina and being subjected to racial prejudices gave him a unique perspective on this issue. “I think any symbol of racial hatred and oppression should not be something the state is endorsing,” Brooks said. “Anything and everything I can do to remove them from society, I’ll do it.” Taney was the fifth chief justice in the history of the U.S. Supreme Court and is remembered most for his decision regarding the Dred Scott case in 1857. Scott was an enslaved African American who said that he and his wife should be granted freedom because they were taken as slaves, by a white owner, to live in Illinois and the Wisconsin Territory for four years. But both those states considered slavery illegal. According to the case’s decision on the Cornell University Law School website, Taney said in the opinion, “Neither Dred Scott himself nor any of his family were made free by being carried into this territory, even if they had been carried there by the owner with the intention of becoming a permanent resident.”Taney grew up in Calvert County and practiced law for over 20 years in Frederick County before becoming a part of the Supreme Court in 1836, according to the Historical Society of Frederick County’s website. Delegate Kathy Afzali, D-Frederick, disagreed with the bill and said there were more important things that the legislature could be doing this session. She also said that there were a lot of historical figures who had prejudices, but who were also in tune with their time.“I honestly think we have parts of our history that are good and then parts that are embarrassing, but he was still a part of our history,” Afzali said.Afzali said that instead of removing the statue, there should be a plaque added to the monument detailing Taney’s decision in the Dred Scott case. However, Brooks said he would consider amending the bill to move rather than destroy the statue.“Let’s put it in a library or something. Memorialize it that way,” Brooks said.last_img read more

first_img ×Actors Reveal Their Favorite Disney PrincessesSeveral actors, like Daisy Ridley, Awkwafina, Jeff Goldblum and Gina Rodriguez, reveal their favorite Disney princesses. Rapunzel, Mulan, Ariel,Tiana, Sleeping Beauty and Jasmine all got some love from the Disney stars.More VideosVolume 0%Press shift question mark to access a list of keyboard shortcutsKeyboard Shortcutsplay/pauseincrease volumedecrease volumeseek forwardsseek backwardstoggle captionstoggle fullscreenmute/unmuteseek to %SPACE↑↓→←cfm0-9Next UpJennifer Lopez Shares How She Became a Mogul04:350.5x1x1.25×1.5x2xLive00:0002:1502:15 Gateley exited A+E Networks’ Lifetime about a year ago. Prior to joining Lifetime in 2015, she was partners with Tony DiSanto in the DiGa production banner, which was behind shows including MTV’s “Teen Wolf.” (In 2014, Gateley and DiSanto sold the company to the U.K.’s ITV. Last year DiSanto and financier Matthew J. Bruderman launched Allyance Media Group, encompassing DiSanto’s production company, DiGa Studios, which had previously reacquired the equity interests in DiGa formerly held by ITV.)Before DiGa, Gateley was a programming exec at MTV where she created “Laguna Beach” and co-created and executive produced its spinoffs, “The Hills” and “The City,” and shepherded other shows for the cable net including “Jersey Shore,” “16 & Pregnant,” “Teen Mom” and “America’s Best Dance Crew.” Gateley had a previous stint at Lifetime prior joining MTV in 2003.At Spotify, Gateley is reuniting with chief content officer Dawn Ostroff, who was head of programming at Lifetime from 1996-2002, and Courtney Holt, head of studios and video (with whom Gateley worked when they were both at MTV).“I am excited to be back in the music/culture world to tell loud stories with my incredible team and to help invent new executions as I always have, but, most importantly, to join at such a transformative time at Spotify on the heels of the Gimlet and Anchor acquisitions,” Gateley wrote in the post. “I also get to work with Dawn Ostroff and Courtney Holt, both former colleagues and two of the smartest people I know.” Spotify has tapped longtime TV exec Liz Gateley, most recently head of programming at Lifetime, as head of creative development focused on original podcast content.Gateley will oversee Spotify’s original content development teams in New York and Los Angeles and the company’s expansion into podcasting including in comedy, sports, YA fiction and scripted, news/documentaries and additional music formats. She announced her new role Thursday in a post on LinkedIn.Her hiring comes after Spotify earlier this month launched a big new push into podcasting, spending almost $340 million to acquire producer Gimlet Media and services platform Anchor. The streaming-audio giant said it planned to invest up to $500 million in podcasting in 2019 (including what it paid for for Gimlet and Anchor).center_img Popular on Variety last_img read more

first_imgIn the prokaryotic respiratory chain, the situation is more complicated. A complete pathway of electron flow has not yet been determined in the cell type due to its complexity. It is therefore necessary to understand the complete structure of a “supercomplex” involved during bacterial electron transfer to assist the goal. In the study, the researchers extracted and purified the complex from M. smegmatis to visualize the architecture using cryo-electron microscopy (cryo-EM) at a resolution of 3.5 Å. The structure provided crucial insights into the mechanism of direct electron transfer within a respiratory supercomplex. The dimensions of the supercomplex were in the range of 200 x 70 x 120 Å, in a symmetrized linear architecture completely different from previously reported respiratory supercomplexes. By composition, the linear dimeric CIV1-CIII2-CIV1 was arranged such that individual CIVs flanked the central CIII dimer on either side. The information revealed a direct link between enzymes during electron transfer, representing a new mode of respiratory chain catalysis. The detailed structural findings have potential to assist with antimycobacterial drug discovery efforts. The architecture of the supercomplex in its entirety: The cartoon representation shows the menaquinone/menaquinol (MK) in bright green colored solid spheres and phospholipids as yellow sticks. Credit: Science, doi: 10.1126/science.aat8923 The authors revealed the cryo-EM structure of a CIII-CIV respiratory supercomplex of the M. smegmatis bacteria. The intra-complex electron transfer pathway ranged from quinol oxidation in CIII to oxygen reduction in CIV. The results showed a new mechanism for bifurcating electron transfer to ensure completion of the Q cycle (the net movement of protons across a lipid bilayer) for energy transduction. The association of a superoxide dismutase in the architecture of the system can protect against oxidative damage by reactive oxygen species (ROS). The architecture of quinone binding sites also provided a framework for future studies in structure-based antimicrobial drug discovery. Study shows how bacteria guide electron flow for efficient energy generation In a recent study conducted by Hongri Gong and colleagues, a respiratory supercomplex was isolated from the bacterium Mycobacterium smegmatis, and its structure was visualized at a resolution of 3.5 Å using cryo-electron microscopy (cryo-EM). The bacterium is a close relative to M. tuberculosis and a popular model used to study many other bacterial species. The detailed structure revealed how electrons were transferred in the cell in a process hitherto unseen. Respiration in Actinomycetes and overall architecture of the Mycobacterial respiratory machine CIII2CIV2SOD2. A) The respiratory electron transfer chain in Actinomycetes (left) and the 5 major prokaryotic cytochrome c pathway variants with the organization schemes in representative organisms (right). The M. smegmas cytochrome c pathway has its cytochrome c fused with complex III to form a bcc-type complex III that interacts with the aa3-type complex IV to assemble into a CIII-CIV supercomplex. MK = menaquinone/menaquinol. B) The entire architecture of the bcc-aa3 type respiratory CIII-CIV Supercomplex from M. smegmatis. The cryo-EM map of the supercomplex shows a linear 2-fold dimerized form of CIV1-CIII2-CIV1 with dimensions 200 x 70 x 120 Å. CIII is seen in orange, CIV is magenta and the association factors PRSAF1 in green, LpqE in blue and SOD in grey. C) Cartoon representation of the side view of the supercomplex (top) and a cross-sectional view (bottom). The MK is presented as bright green solid spheres and phospholipid as yellow sticks. In the cross-sectional view at the bottom, the boundaries of CIII, CIV and association factor PRSAF1 are depicted in dashed lines in color (orange for CIII, magenta for CIV and green for PRSAF1). Credit: Science, doi: 10.1126/science.aat8923. More information: Hongri Gong et al. An electron transfer path connects subunits of a mycobacterial respiratory supercomplex, Science (2018). DOI: 10.1126/science.aat8923Eric F. Pettersen et al. UCSF Chimera?A visualization system for exploratory research and analysis, Journal of Computational Chemistry (2004). DOI: 10.1002/jcc.20084 During cryo-EM analysis, the researchers used uranyl acetate (1 percent w/v) for negative staining, using 5 µl of the supercomplex sample at a concentration of 0.05 mg/ml, images were taken on a FEI Tecnai Spirit microscope operating at 120 kV for initial model building. The acquired images were processed using a low-resolution reconstruction of the supercomplex from 53 micrographs of the negative stained sample. For complete reconstruction of the supercomplex, the authors manually selected 7,600 micrographs from 8,200 original micrographs during cryo-EM image processing. All figures in the study were created using PyMOL or UCSF chimera. The composition and structure of CIV. Cartoon representation of the complex IV. The phospholipids are shown as yellow sticks. Credit: Science, doi: 10.1126/science.aat8923 This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. © 2018 Science X Network Typically, chemical energy to synthesize adenosine triphosphate (ATP) and power cellular reactions is extracted during cellular respiration by coupling the oxidation of an energy source (sugars, fatty acids and amino acids) and the reduction of an electron acceptor (oxygen, sulfur, nitrate and sulfate). In aerobic cellular respiration, energy is extracted from electron donors to the terminal acceptor, oxygen, via the electron transport chain (ETC) to create a transmembrane proton gradient known as a proton motive force (PMF) that drives ATP synthesis. The new results now published in Science reveal a direct link for electron transfer between enzymes to represent a new mode of respiratory chain catalysis. Quinones and cytochromes are two types of electron carriers in ETCs used to shuttle electrons to and from large macromolecular structures embedded in the membrane. Four membrane oxidoreductases are involved in the mitochondrial respiratory chain for electron transfer. These include complex I (NADH:ubiquinone oxidoreductase, CI), complex II (succinate:ubiquinone oxidoreductase, CII), complex III (bc1-type ubiquinol:cytochrome c oxidoreductase, bc1-type CIII) and complex IV (aa3-type cytochrome c oxidase, aa3-type CIV). By function, CIII can oxidize ubiquinol to ubiquinone and pass the electrons to soluble cytochrome c. Electrons are then shuttled to CIV, where oxygen is reduced to water. The transmembrane PMF is generated by proton pumping in CI, CIII and CIV. The composition and structure of CIII dimer. The menaquinone/menaquinol is presented as bright green solid spheres and the phospholipids are yellow sticks. Credit: Science, doi: 10.1126/science.aat8923 Journal information: Science During bacterial cell culture experiments, the authors used an M. tuberculosis-like hydrogen peroxide-resistant M. smegmatis mutant strain. The cells were cultured and the membrane isolated as previously described. After cell culture, harvesting and cell lysis, cell membrane pellets were harvested to extract respiratory supercomplexes. The supercomplexes were characterized using optical spectroscopy, mass spectroscopy and 3,3′-diaminobenzidine (DAB) staining. To identify heme groups, selected fractions were analyzed by recording spectra before and after reduction with dithionate as described previously. The purified samples were analyzed using native mass spectroscopy to investigate the architecture and the individual structural components were analyzed using previously established protocols. Citation: Ready for its close-up—a bacterium’s electron transport pathway (2018, November 5) retrieved 18 August 2019 from https://phys.org/news/2018-11-ready-close-upa-bacterium-electron-pathway.html Explore furtherlast_img read more

first_imgTwo-and-a-half years after he met with a crippling road accident that cut short his career, veteran Malayalam film actor Jagathy Sreekuamar was awarded Rs 5.90 crore by a private insurance company as compensation. The documents regarding the award of compensation was handed over to the wheelchair-bound actor at his residence here by top officials of the insurance company. The actor and his family members had earlier clinched an agreement with the insurance company at an Adalat conducted by the District Legal Services Authority. Jagathy met with a major road accident while travelling in a car at Thenhippalam near Kozhikode in March 2012, when he was on his way to a shooting location at Kodagu in Karnataka. This followed prolonged treatment in hospitals.last_img

first_imgKolkata: Members of the Matua community staged demonstrations at different parts of the state, protesting against the exclusion of the names of 40 lakh Bengalis in the final draft of Assam’s National Register of Citizens (NRC).It may be mentioned that Chief Minister Mamata Banerjee had stated that with exclusion of their names, the people have turned refugees in their own country and it is the outcome of BJP’s “vote politics”.Stating that the “people are being isolated”, the Chief Minister had said: “BJP’s divide and rule policy to isolate people has led to the situation.” Also Read – Rain batters Kolkata, cripples normal lifeThe Chief Minister, who is in Delhi at present, criticised the government at the Centre over Assam’s NRC issue.The protest started at around 8 am and continued till noon. People from the community carried out agitation mainly in North 24-Parganas and Nadia districts. They also put up a symbolic railway blockade at Panihati Railway Station.With the protest, there was a disruption in train services since morning at different stations of Sealdah Division. Also Read – Speeding Jaguar crashes into Mercedes car in Kolkata, 2 pedestrians killedThere were obstructions at various stations of the Sealdah-Ranaghat Main Line, Sealdah-Bangaon, Sealdah-Dankuni and Barasat Hansnabad sections.There were obstructions at Sandalia, New Barrackpore, Gobardanga, Dum Dum, Belghoria, Ichhapur, Kanchrapara, Chakdaha, Halisahar, Bira, Naihati, Titagarh, Palta, Baranagar, Sodepur, Dankuni, Machlandapur, Habra, Ashoknagar, Duttapukur, Bamangachi, Barrackpore, Badkulla,Bongaon, Ranaghat, Akaipur, and Thakurnagar. The train services resumed at around 12 noon. There was cancellation of 51 local trains of Sealdah Division.People from the Matua community also protested against the move by putting up a roadblock at Bagnan in Howrah on National Highway 6, demanding immediate steps so that people do not turn refugees in their own country.There were long queues of vehicles on the national highway. It took some time to normalise the traffic movement after the demonstration was withdrawn.Commuters were greatly inconvenienced as train services in the main section were badly hit. The demonstration was lifted and train services became normal from noon.last_img read more